Sequence Prediction

Visualise and score TF binding affinity across arbitrary DNA sequences. BoltzNet predicts binding strength for all 124 E. coli TF models.

1. File Uploads *

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Upload a FASTA file containing the DNA sequences to score.
Format rules:
  • All sequences must be the same length, between 101 bp and 1500 bp.

Maximum 1,000,000 sequences per score job.
Supported formats: .fa, .fasta, .fna. Up to 1,000,000 sequences, 101–1500 bp each, all the same length. Example

2. Score Settings

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Limit scores to a specific set of TFs.
Type a TF name and press Enter to add it. Each TF appears as a removable tag.
Leave empty to get scores for all 124 TF models.
Leave empty to score all 124 TFs.
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Max binding site score
Score of the single strongest binding position anywhere in the sequence.
Full sequence score
Sum of binding energies across all positions on both strands. Reflects total binding potential across the whole sequence.
Scores are normalized against the E. coli genome so transcription factors are directly comparable.

3. Notification Email *

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The results link will be sent to this address.
Please enter a valid email address (e.g., name@example.com).
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